A Novel Specific Small Molecule Peptide for Small Cell Lung Cancer
- VernacularTitle:一种特异性识别小细胞肺癌细胞的小分子肽
- Author:
Linlang GUO
;
Ying GUO
;
Derick LAU
;
Sha XIAO
;
Yinchao XU
;
Hong SHEN
- Publication Type:Journal Article
- Keywords:
small molecule peptide, combinatorial chemistry, small cell lung cancer
- From:
Progress in Biochemistry and Biophysics
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Screen small molecule peptide specific binding to small cell lung cancer cell (DMS53) was screened by using the "one-bead one-peptide" combinatorial technology. Thirty two positive beads binding to DMS53 were totally obtained after primary screening. Consensus peptide sequences of cXNGRXXc and cNGRXXXc were identified by amino acid sequencing in ten beads. Three representative peptides were re-synthesized on beads. Secondary screening showed that cell adhesion percentage of cFNGRQQc to DMSS was higher than the other two peptides. cFNGRQQc was further studied for cell specificity, alanine scanning and site-directed deletion. The results showed that cFNGRQQc is specific for promoting cell adhesion to DMS53 but not to other human cell lines. Both motif of -NGR- and the length of six peptide of cFNGRQQc structure are important for DMS53 attachment. In an antibody or peptide blocking assay, cell adhesion of DMS53 to peptide bead was not inhibited by antibodies or peptides including anti-integrin, E-cadherin, NCAM and ICAM. The binding site on DMS53 surface for cFNGRQQc peptide need to be proven in the future.
