Mutations in E154 of Diphtheria Toxin (DT) and Their Biologic Activity
- VernacularTitle:白喉毒素E154位突变及生物活性评价
- Author:
Chuan GAO
;
Huifang WANG
;
Jing ZHANG
;
Yunyang SONG
;
Yalan HU
- Publication Type:Journal Article
- Keywords:
diphtheria toxin, quantum chemistry, mutant, LD50, IC50
- From:
Progress in Biochemistry and Biophysics
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
According to the results of quantum chemistry calculation and the present research status in the relationship between the structures and the functions of DT, the E154 in DT catalyzing domain was mutated to aspartic acid and arginine in order to study the effects of the alteration on the biological activities. By means of gene site-direct mutation, two mutated genes were prepared and the high performance expression was obtained in E.coli system. The results of toxcity studies indicated that the acute toxicity in guinea pig and cytotoxicities of mutant E154D increased slightly in compared with those of recombination wild toxin, and contrarily, those of E154R decreased obviously.
