A Novel Method for Quickly Cloning Genes With Multiple DNA Fragments Using Isoschizomer-heterotail Restriction Endonuclease (IHRE)
- VernacularTitle:利用同序异尾限制性内切酶快速克隆多基因片段的新方法
- Author:
Guangying DU
;
Naishuo ZHU
- Publication Type:Journal Article
- Keywords:
molecular biology, isoschizomer-heterotail restriction endonuclease (IHRE), cloning, gene with multiple DNA fragments
- From:
Progress in Biochemistry and Biophysics
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A novel method for quickly cloning genes with multiple DNA fragments--one step cloning technique using isoschizomer-heterotail restriction endonuclease (IHRE) is described. Up to six DNA segments are ligated by using only one restriction endonuclease in this method. Comparing with routine method,it is simple, fast, economical and generates products with higher purity and achievement. Light chain of human enterokinase, DNA multi-epitope vaccine to HSV2 have been designed and successfully constructed via this method.
