cDNA cloning and prokaryotic expression of human neural RNA binding protein HuC
- VernacularTitle:人神经系统特异表达RNA结合蛋白HuC cDNA的克隆、表达及纯化
- Author:
Hua QIANG
- Publication Type:Journal Article
- Keywords:
RNA binding protein HuC;
cDNA cloning;
prokaryotic expression;
purification
- From:
Basic & Clinical Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone human neural RNA binding protein HuC cDNA,express and purify the recombinant human HuC(Hu antigen C)protein in E.coli.Method Human HuC cDNA was cloned by RT-PCR.HuC cDNA was inserted into the prokaryotic expression vector pGEX-4T-3.The recombinant protein HuC was expressed in E.coli BL-21,and purified by the GST Sepharose 4B affinity column.Results The(62 ku) recombinant GST-HuC fusion protein was obtained.Conclusion The recombinant human HuC protein was successfully prokaryotic expressed and purified.
