Effect of blocking BRCAA1 gene with siRNA on proliferation of MCF-7 cells and expression of Rb gene
- VernacularTitle:siRNA封闭BRCAA1基因对乳腺癌细胞MCF-7增殖和Rb基因表达的影响
- Author:
Hao YANG
;
Daxiang CUI
;
Qing LI
;
Tuo HUANG
;
Feng GAO
;
Rong HE
;
Bifeng PAN
;
Jun SHAO
;
Xiaogang YOU
;
Fengtao LIU
- Publication Type:Journal Article
- Keywords:
siRNA;
BRCAA1 gene;
breast carcinoma;
Rb gene
- From:
Chinese Journal of Cancer Biotherapy
1995;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of blocking BRCAA1 gene expression with siRNA on the proliferation of tumor cell line MCF-7 and Rb gene expression.Methods:RNAi was employed to specifically knock down BRCAA1 expression.MCF-7 cells were transfected with complexes constructed with lipids and chemically synthesized Pre-designed anti-BRCAA1 siRNAs.The total RNA was isolated and reversely transcribed after 48 h.The expressions of BRCAA1 and Rb mRNA were determined by Real-Time PCR.Results:Compared with negative control,transfected MCF-7 cells had a 42.3% decrease in expression of BRCAA1 mRNA and an 11.1% increase in Rb mRNA expression.The inhibitory rate of MCF-7 cells proliferation was(81.6?6.1)%.Conclusion:There may be some antagonistic effect between BRCAA1 gene and Rb gene in proliferation of tumor cells,which provides a potential target for anti-tumor gene therapy.
