A method for preparation of saturated oligosaccharides from alginate
- VernacularTitle:一种饱和褐藻胶寡糖的制备方法
- Author:
Hongrong ZHANG
;
Changyun WANG
;
Bin LIU
;
Yuxi WEI
;
Bingui WANG
;
Meiyu GENG
;
Huash GUAN
- Publication Type:Journal Article
- Keywords:
alginate;
saturated alginate oligosaccharides;
preparation;
acid hydrolysis
- From:
Chinese Journal of Marine Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To report a method for preparation of saturated oligosaccharides from al- ginate. Methods The alginate was degraded by acid hydrolysis, followed by fractionation at pH 2.85 resulting in the homopolyguluronic acids (PG) and homopolymannuronic acids (PM). The alginate-derived homopolymers were further degraded by acid hydrolysis at pH 3.8, 120 ℃ for 3 h, and at pH 4, 120 ℃ for 4 h, respectively. The resulted acidic hydrolysates were separated by gel permeation chromatography to get oligosaccharide fractions, and the oligosaccharides were obtaind by repeated purification on gel chromatography. The structures of the oligosaccharides were characterized by analyses of infra-red spectroscopy (IR), nuclear magnetic resonance spectrometry (NMR), and mass spectrometry (MS). Results It was showed that the derived oligosaccharides were in saturated structures with the same structure characteristics as the original alginate macromolecule. Conclusion The method des cribed in this paper is a effective and convenient approach for the preparation of low-molecular-weight oligosaccharides from alginate.
