Experimental study on restenosis after percutaneous coronary intervention: effects of fenofibrate on proliferation and apoptosis of cultured human umbilical vein endothelial cells
- VernacularTitle:经皮冠状动脉介入治疗后再狭窄的实验研究——非诺贝特对内皮细胞增殖及凋亡的干预作用
- Author:
Guoju SUN
;
Xiumei XIE
;
Xiaobin CHEN
- Publication Type:Journal Article
- Keywords:
Fenofibrate;
Endothelium;
Proliferation;
Apoptosis
- From:
Chinese Journal of Interventional Cardiology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the cell model of restenosis in vitro and investigate the effects of fenofibrate on the prevention and treatment of the restenosis.Methods Human umbilical vein endothelial cells(HUVECs) were cultured in vitro.The study was designated to 5 groups:(1) control group,(2) LPC group,(3) low-concentration fenofibrate(10 ?mol/L) group,(4) midium-concentration fenofibrate(50 ?mol/L) group,and(5) high-concentration fenofibrate(100 ?mol/L) group.Proliferation and apoptosis of HUVECs were assessed by MTT assay,flow cytometry(FCM) and fluorescence microscopy respectively.Results Compared with the control group,LPC could inhibit the growth and induce apoptosis of HUVECs as well as decrease NO production in HUVECs.Fenofibrate could increase the proliferation and decrease the apoptosis of HUVECs,and also enhance NO production of HUVECs.Conclusion Fenofibrate could improve proliferation and reduce apoptosis and enhance NO production of HUVECs through lysophosphatidylcholine,which may be an important route for fenofibrate to prevent and treat the restenosis after percutaneous coronary intervention.
