Increased ICAM-1 expression on bronchial epithelial cells induced by eosinophils
- VernacularTitle:嗜酸粒细胞对支气管上皮细胞表达细胞间黏附分子1的诱导作用
- Author:
Chengbin WANG
;
Zhenguo HUANG
;
Weiji YE
;
Yaping TIAN
;
Weiji LIN
- Publication Type:Journal Article
- Keywords:
Bronchi;
Epithelial cells;
Granulocytes;
Cell adhesion molecules
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of human bronchial epithelial cells (BEAS-2B cells ) coculture together with human eosinophils on intercellular adhesion molecule (ICAM-1) expression on BEAS-2B cell surface.Methods BEAS-2B cells cocultured contact with eosinophils for 4 h and 12 h,total RNA was extracted and ICAM-1 gene in BEAS-2B cells were analyzed by reverse transcription polymerase chain reaction (RT-PCR); ICAM-1 protein on BEAS-2B cell surface was assayed by fluorescent antibody in flow cytometry.Results ICAM-1 gene expression in BEAS-2B cells was upregulated and ICAM-1 protein on BEAS-2B cell surface increased by coculture with eosinophils.Conclusion Coculture of BEAS-2B cells and eosinophils might induce BEAS-2B cells to express ICAM-1.
