Expression of a new qnr gene subtype and mechanism of multidrug resistance
- VernacularTitle:一个新qnr基因亚型的克隆表达及其多重耐药机制的研究
- Author:
Tao LI
;
Zizhong XIONG
;
Yuanhong XU
- Publication Type:Journal Article
- Keywords:
Genes;
Quinolones;
Cloning,molecular;
Multidrug resistance associated proteins
- From:
Chinese Journal of Laboratory Medicine
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biologic characteristics of the new qnr gene subtype and multi-drug resistant mechanism in a clinical isolate of Klebsiella oxytoca.Methods We cloned and expressed the qnr gene,?-lactamase and integrase genes were detected by PCR.Results Susceptibility of transformant containing qnr gene against common fluoroquinolones was 3~25 times lower than recipient stain,but drug-resistance was 4~256 times lower than the clinical isolate.KLUC-1,TEM-1 and OXA-30 genes were also found in the isolate.Conclusions qnr gene can raise the drug-resistance to fluoroquinolones slightly. There are multiple drug-resistant genes in the strain containing the qnr gene.
