Effects of MPP~+ on expression of inhibin,activin and its receptorⅡA and cell ability in PC12 cells of rats
- VernacularTitle:1-甲基-4-苯基吡啶离子对大鼠嗜铬细胞的抑制素、活化素及其受体ⅡA表达以及细胞活力的影响
- Author:
Nan LIU
;
Yuping JIANG
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
PC12 cells;
Parkinson's disease;
1-methyl-4-phenyl pyridinium;
activin
- From:
Journal of Clinical Neurology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of MPP+ on the expression of inhibin, activin and its receptor ⅡA and cell ability in PC12 cells of rats.Methods The changes of expression of activin?A, activin?B, inhibin? and receptorⅡA mRNAs were assayed by RT-PCR, the change of cell viability was detect by tyrpan blue exclusion method in cultured PC12 cells at 3 h,6 h,12 h,24 h after MPP+ was added and compared with control groups.Results The expression of activin?A, activin?B and activin receptorⅡA mRNAs in PC12 cells were down-regulated significantly after MPP+ administrated at every time point while the expression of inhibin? mRNA remained unchanged. The cell viability decreased at the points of 12 h and 24 h after MPP+ administrated.Conclusion MPP+ may cause the injury of PC12 cells by downregulating the expression of activin and its receptor.
