Cloning of Ribosomal protein L6-encoding gene and constructing of sense and antisense eukaryotic expression vectors
- VernacularTitle:人核糖体蛋白L6编码基因的克隆及正反义真核表达载体的构建
- Author:
Hong CHEN
- Publication Type:Journal Article
- Keywords:
Ribosomal protein L6;
Cloning;
Vectors
- From:
Journal of Medical Postgraduates
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between ribosomal protein L6 and MDR of(K562/A02) cell by cloning of RPL6-encoding gene and constructing of sense and antisense eukaryotic expression vectors. Methods: RPL6 cDNA was obtained from its mRNA and amplified by RT-PCR.Results:The cDNA encoding RPL6 from K562/A02 cell was successfully amplified and PCR product was sequenced and identified to be consistent with RPL6 cDNA reported in GenBank.Recombination plasmids containing both sense and antisense RPL6 cDNA were identified by gel electrophoresis analysis(using) restriction endonuclease. Conclusion: RPL6-encoding gene has been successfully cloned and sense and antisense eukaryotic expression vectors constructed.
