Immunomodulatory effects of lactobacillus on dust mite sensitized murine model
- VernacularTitle:乳酸菌对尘螨致敏哮喘小鼠模型的免疫调节作用
- Author:
Li LI
;
Baodan YU
;
Jun XU
- Publication Type:Journal Article
- Keywords:
Lactobacillus;
Dust mites;
Asthma
- From:
Journal of Medical Postgraduates
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the immunomodulatory capability to allergic asthma between orally taken Lactococcus lactis and Lactobacillus Lactobacillus plantarum in dust mite-sensitized mice. Methods: C57BL/6 mice were divided into four groups;Group N,M,L and P.Mice in M,Land P groups were sensitized with 2 % mite extract on day 0,7and 14 and challenged with 4% mite extract on day 21,22,23 and 24.Meanwhile we fed Lactococcus lactis and Lactobacillus plantarum to mice in L and P group respectively from each day of sensitization on as well as from the first day of challenge on,continuing for five days.Mice in N and M group were treated with saline as control.Histopathologic examination in the lung tissues of the mice was performed at day 3 after the last challenge.Cell count and classification,and Th1/Th2 cytokine levels in BALF were measured simultaneously.Isolated splenocytes from the mice were subjected to the detection of cell proliferation by BrdU incorporation in response to the mite extract.Using ELISA,the levels of Th1/Th2 cytokines in the supernatant of mite-stimulated splenocytes were measured. Results: Pulmonary allergic inflammation with eosinophilic infiltration as well as increased levels of IL-4 and IL-5 in BALF were observed in mice sensitized/challenged with mite extract(Group M).Pulmonary inflammation was decreased significantly in group L concomitant with down regulated levels of IL-4 and IL-5,total cell count and the percentage of eosinophils in BALF(P
