Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients.
- Author:
Su Mi BAE
1
;
Hyun Jin MIN
;
Guo Hua DING
;
Sun Young KWAK
;
Young Lae CHO
;
Kye Hyun NAM
;
Choong Hak PARK
;
Yong Wan KIM
;
Chong Kook KIM
;
Byoung Don HAN
;
Young Joo LEE
;
Do Kang KIM
;
Woong Shick AHN
Author Information
1. Cancer Research Institute, The Catholic University of Korea, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cervical cancer;
Squamous cell carcinoma;
Two-dimensional polyacrylamide gel electrophoresis;
Matrix assisted laser desorption/ ionization-time of flight mass spectrometer
- MeSH:
Annexin A2;
Apolipoproteins;
Biomarkers;
Carcinoma, Squamous Cell;
Cervix Uteri;
Databases, Protein;
Electrophoresis, Polyacrylamide Gel;
Epithelium;
Female;
Glutathione Transferase;
HSP27 Heat-Shock Proteins;
Humans;
Hydrogen-Ion Concentration;
Keratin-19;
Mass Screening;
Muscle, Smooth;
Phosphopyruvate Hydratase;
Uterine Cervical Neoplasms*
- From:Cancer Research and Treatment
2006;38(2):99-107
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Screening in cervical cancer is now progressing to discover candidate genes and proteins that may serve as biological markers and that play a role in tumor progression. We examined the protein expression patterns of the squamous cell carcinoma (SCC) tissues from Korean women with using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. MATERIALS AND METHODS: Normal cervix and SCC tissues were solubilized and 2-DE was performed using pH 3~10 linear IPG strips of 17 cm length. The protein expression was evaluated using PDQuest 2-D software(TM). The differentially expressed protein spots were identified with a MALDI-TOF mass spectrometer, and the peptide mass spectra identifications were performed using the Mascot program and by searching the Swiss-prot or NCBInr databases. RESULTS: A total of 35 proteins were detected in SCC. 17 proteins were up-regulated and 18 proteins weredown-regulated. Among the proteins that were identified, 12 proteins (pigment epithelium derived factor, annexin A2 and A5, keratin 19 and 20, heat shock protein 27, smooth muscle protein 22 alpha, alpha-enolase, squamous cell carcinoma antigen 1 and 2, glutathione S-transferase and apolipoprotein a1) were protein previously known to be involved in tumor, and 21 proteins were newly identified in this study. CONCLUSION: 2-DE offers the total protein expression profiles of SCC tissues; further characterization of these differentially expressed proteins will give a chance to identify the badly needed tumor-specific diagnostic markers for SCC.
