Randomly Amplified Polymorphic DNA in Epidemiological Study of Pseudomonas aeruginosa
- VernacularTitle:随机扩增多态性DNA分型法在铜绿假单胞菌流行病学研究方面的应用
- Author:
Yibing PENG
;
Mingjie XIANG
;
Jian ZHANG
;
Yuhua JI
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Randomly amplified polymorphic DNA
- From:
Chinese Journal of Nosocomiology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a fingerprinting method by randomly amplified polymorphic DNA.Epidemiological study was carried out on Pseudomonas aeruginosa in Ruijin Hospital. METHODS To obtain optimum scheme on reaction system for randomly amplified polymorphic DNA(RAPD) of P.aeruginosa. RESULTS P.aeruginosa strains isolated from the same ward shared the same RAPD fingerprint type,except for pulmonary ward.Different ward was with different fingerprint type. CONCLUSIONS Prevalent strain was not found in the whole hospital,but within ward exists hospital-acquired infection phenomenon.
