The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-alpha and IL-1beta Secretion.
10.4046/trd.2006.60.5.554
- Author:
Tian Zhu LI
1
;
Soo Jin LEE
;
Se Jong PARK
;
Byung Joon CHANG
;
Jong Hwan LEE
;
Kil Soo KIM
;
Myoung Heon LEE
;
Nong Hoon CHOE
Author Information
1. Konkuk University College of Veterinary Medicine, Seoul, Korea. nojamaji@hanmail.net
- Publication Type:Original Article
- Keywords:
Particulate matter (PM);
Alveolar macrophages;
Tumor necrosis factor-alpha (TNF-alpha);
Interleukin 1beta (IL-1beta)
- MeSH:
Animals;
Asthma;
Blotting, Western;
Bronchitis, Chronic;
Coloring Agents;
Enzyme-Linked Immunosorbent Assay;
Fibrosis;
Lung Diseases;
Macrophages;
Macrophages, Alveolar*;
Rats;
Tumor Necrosis Factor-alpha*
- From:Tuberculosis and Respiratory Diseases
2006;60(5):554-563
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear. METHODS: This study examined the effects of PM exposure on the secretion of TNF-alpha and IL-1beta in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM (5~20 microgram/cm2), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted TNF-alpha and IL-beta in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the Lab-Tek(R) chamber slides were stained with immunocytochemical stains. RESULTS: PM induced TNF-alpha and IL-1beta secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types. CONCLUSION: PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of TNF-alpha and IL-1beta.
