Osteopontin promotes adhesion and migration of hepatic stellate cells and FAK phosphorylation
- VernacularTitle:OPN促进大鼠肝星状细胞黏附、迁移及黏着斑激酶活性
- Author:
Caixia HU
;
Huiqing JIANG
;
Xiaolan ZHANG
;
Li LIU
- Publication Type:Journal Article
- Keywords:
hepatic fibrosis;
hepatic stellate cells;
adhesion;
migration;
focal adhesion kinase
- From:
Basic & Clinical Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of osteopontin(OPN) on the adhesion and migration of hepatic stellate cells(HSC) and focal adhesion kinase(FAK)phosphorylation.Methods The proliferation of HSCs was determined by MTT.The adhesion rates were shown by toluidine blue colorimetric assay.The HSC migration rates were examined by HE staining.The expression of FAK phosphorylation was examined by Western blotting analysis.Results ①Compared with control group,OPN at concentrations of 8 mg/L,16 mg/L and 32 mg/L promoted the adhesion of HSCs(P
