Application of RNA interference on gene expression of survivin
- VernacularTitle:RNA干扰技术用于survivin基因表达的研究
- Author:
Bo SHEN
;
Guizhi JU
;
Yang LIU
- Publication Type:Journal Article
- Keywords:
survivin;
RNA interference;
gene expression
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct pSUPER-SVV vector and obtain its expression in HeLa cells,in order to interfere the expression of survivin.Methods Oligonucleotide sequences specific for survivin were designed for RNA interference(RNAi),after annealed they were ligated to pSUPER.basic vector.After sequencing,the vector of pSUPER-SVV was transfected into HeLa cells by LipofectAmine.The expression of survivin was detected by RT-PCR and FCM.Results The RNAi expression vector of survivin was constructed successfully,which depressed the expression of survivin efficiently.Survivin was significantly depressed on the level of mRNA in HeLa cells compared with the control groups(P
