Construction of prokaryotic expression vector harboring different domains of STAT6
- VernacularTitle:STAT6分子不同功能域原核表达载体的构建
- Author:
Yanbo SUN
;
Hongyan SHI
;
Jinghua LI
- Publication Type:Journal Article
- Keywords:
signal transducer and activators of transcription;
Janus activated kinase;
T lymphocytes;
interleukin-4
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of different domains of STAT6 expressed by the vector pGEX-6p-2 on signal transduction.Methods Different domains of STAT6 were amplified by PCR technique and ligated respectively into the prokaryotic expression vector.Recombinant DNA was transformed into HB101 cells.Transformants were lysed by supersonic method and lysates run on SDS-PAGE.Results Western blotting analysis showed a high level expression(60 000,56 000 and 50 000) of different domains of STAT6 SHST,DST and NST,and the empty vector only had GST expression.Conclusion Expression vector pGEX-6p-2 can be used for high level expression of different domains of STAT6.
