Effect of As_2O_3 on mouse bone marrow hemopoietic microenvironment
- VernacularTitle:三氧化二砷对小鼠骨髓造血微环境的影响
- Author:
Fang YANG
;
Wei LI
;
Xiaoxing YUAN
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
As_2O_3;
bone marrow cells;
granulocyte colony stimulating factor;
interleukin-11
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of As_2O_3 on normal mouse bone marrow stromal cells(BMSCs).Methods BMSCs and colony-forming unit-granulocyte macrophage (CFU-GM) were cultured in vitro to observe the quantitative changes of colony-forming unit-fibroblast(CFU-F) and CFU-GM after BMSCs were treated with different concentrations of As_2O_3 respectively;the quantitative changes of CFU-GM supported by BMSCs and granulocyte colony stimulating factor(G-CSF) and IL-11 secreted by BMSCs were also observed after the progenitor cells supported by BMSCs were treated with As_2O_3 for 3,5 and 7 d,respectively.Results The productions of CFU-F and CFU-GM supported by BMSCs were decreased obviously when treated with 7 ?mol?L~(-1) As_2O_3,also the levels of G-CSF and IL-11 secreted by BMSC(P
