Effects of selenium and vitamin E on collagen Ⅰ synthesizing metabolism of cultured cardiac fibroblasts
- VernacularTitle:硒和维生素E对心肌成纤维细胞 Ⅰ型胶原合成代谢的影响
- Author:
Chunsheng MIAO
;
Dan ZHANG
;
Xiangjun LI
- Publication Type:Journal Article
- Keywords:
selenium;
vitamin E;
myocardium;
fibroblasts;
collagen type Ⅰ
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of selenium(Se) and vitamin E(VE) on collagen Ⅰ synthesis in cultured cardiac fibroblasts(CFbs) treated with norepinephrine(NE).Methods CFbs were obtained by the method of enzymatic digestion culture.Cells and culture medium were collected after 48 h,then the hydroxyproline(HYP) and collagen Ⅰ content as well as the expression of ?_1 col1agen mRNA were examined.Results The contents of HYP in Se,VE and Se+VE groups were(64.88?1.25),(47.60?1.59) and(41.34?(1.73) mg?L~(-1)) prot,respectively,all of them were lower than that in NE group(P
