Effects of chronic mthylmercury chloride exposure on activity of protein kinase C in rat cerebellum during development
- VernacularTitle:长期接触低剂量氯化甲基汞对发育阶段大鼠小脑蛋白激酶C活性的影响
- Author:
Jie GUO
;
Xiaoying BI
;
Cuiping LEI
;
Yongjin LI
;
Zhichao LI
- Publication Type:Journal Article
- Keywords:
methylmercury compounds/toxicity;
protein kinase C;
mercury poisoning,nervous system;
animal,laboratory
- From:
Journal of Jilin University(Medicine Edition)
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of methylmercury chloride(MMC) on protein kinase C(PKC) activities in rat developing cerebellum.Methods The animals in experimental groups were fed standard rat chow with(0.75 mg) MMC(ExpⅠ),1.5 mg MMC(ExpⅡ) and 3.0 mg MMC(ExpⅢ)respectively for 90 d before gestation to(30 d) post parturition.Cerebella of pups from each group on postnatal days(PND) 3,7,17,21 and 30 were dissected. All the samples were separated into cytosol and membrane subcellular fractions and assayed for PKC activity by the improved method from Takai′s.Results Membrane and cytosolic PKC activities of pups′ cerebella from certain experimental groups were significantly higher than those of corresponding control group,PKC activities of rats from ExpⅡ,ExpⅢ and PND 3,7,14 in ExpⅠwere significantly higher than those in control group((P
