Comparative study on detection methods of mutations in hepatitis B virus precore and basic core promoter
- VernacularTitle:乙型肝炎病毒前C区及基本核心启动子变异检测方法的比较研究
- Author:
Jingjuan DING
;
Yuehui LIU
;
Mei WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Mutation;
Restriction fragment length polymorphism;
Sequencing
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05). The sequence of five clones from one serum which was identified precore mutation by mPCR-RFLP were all A1896 mutant strains.Another serum identified mixture infection by mPCR-RFLP , one clone was A1896 mutant strain and four were G1896 wild strains.The results of mPCR-RFLP were verified by cloning.Conclusions Comparison with sequencing, the mPCR-RFLP method is simple,accurate and can be used in large-scale surveys and clinical research.
