Function of GPⅠa/Ⅱa in the platelet-collagen interactions
- VernacularTitle:血小板-胶原相互作用过程中GPⅠa/Ⅱa的功能
- Author:
Zhuo ZHANG
;
Yulong CONG
;
Zhiguang TU
- Publication Type:Journal Article
- Keywords:
Blood platelets;
Collagen;
Flow cytometry
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of GPⅠa/Ⅱa in the platelet-collage adhesion and the platelet granules release induced by collagen. Methods With the flow cytometry, in either presence or absence of the anti-GPⅠa/Ⅱa antibody: (1) PE-labeled anti-CD42b antibody was used for selecting platelet and FITC fluorescence intension was detected during the course of platelet adhered to FITC-labeled collagen. (2) PerCP-labeled anti-CD61 antibody was used for selecting platelet and detecting CD62P with PE-labeled CD62P antibody during platelet granule release induced by collagen. Results Anti-GPⅠa/Ⅱa antibody inhibited the platelet-collagen adhesion, especially the activated platelet-collagen adhesion (P0.05). Conclusion GPⅠa/Ⅱa plays an important role in platelet-collagen adhesion. Blocking GPⅠa/Ⅱa can decrease the platelet-collagen adhesion, but has no effects on platelet granule release reaction.
