Quantitative detection of cytomegalovirus DNA by real-time PCR in transplant recipients
- VernacularTitle:荧光定量聚合酶链反应检测移植受者的巨细胞病毒DNA载量
- Author:
Xiaotao ZHAO
;
Zheng ZHANG
;
Yanrong ZHANG
;
Damin LIU
;
Yuanyuan SUN
- Publication Type:Journal Article
- Keywords:
Cytomegalovirus;
Polymerase chain reaction;
Fluorescence;
Transplantation
- From:
Chinese Journal of Laboratory Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the advantages of cytomegalovirus (CMV) real-time PCR, to monitor CMV infection in a population of transplantation recipients in comparison with the qualitative PCR method.Methods 150 plasma samples and leukocytes samples collected from 59 bone marrow transplant recipients and 9 liver transplant recipients were tested by qualitative CMV PCR assay in parallel; Real-time CMV PCR using Roche Light Cycler was performed with 150 plasma samples and 54 control plasma samples.Results Using real-time PCR as the reference standard, the sensitivity and specificity of the qualitative CMV PCR assay with leukocytes samples were 62.5% and 95.5%, with plasma samples were 57.5% and 99.1% respectively. The recipients with the level of CMV DNA over 5?10~3 copies/ml by real-time PCR had higher percentage of developing CMV disease than those below 10~3 copies/ml.Conclusion The quantitative detection of CMV DNA by real-time PCR with plasma is a rapid, specific and sensitive method to monitor CMV infection in patients after transplantation and to guide antivirus therapy.
