Nestin expression in fibroblasts of adult human dermis
- VernacularTitle:巢蛋白在人真皮成纤维细胞中的表达
- Author:
Wei QIAO
;
Dongming CHEN
;
Rongsheng QIN
- Publication Type:Journal Article
- Keywords:
Nestin;
Dermis;
Fibroblast;
Precursor
- From:
Chinese Journal of Minimally Invasive Surgery
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of nestin in fibroblasts of adult human dermis.Methods A total of 6 samples of normal human skin were collected.Immunohistochemistry and immunocytochemistry staining were used to detect the nestin expression in adult human dermis and in cultured fibroblasts of different passages(3rd,5th,7th,10th,and 12th passages,respectively) in vitro.Nestin positive cells were counted to evaluate the expression quantity and intensity.Results There were 103.3?67.4 fibroblasts per square millimeter(mm~2) of adult human dermis,and the nestin positive fibroblasts accounted for(9.5?3.0)% of total amount.The amount of nestin positive fibroblasts in vitro was obviously higher than that in vivo.Significant difference was observed in the amount of nestin expression among different passages(P
