The Effect of alpha-MSH on Long-term Histologic Changes after Acute Ischemic Kidney Injury in Rats.
- Author:
So Young LEE
1
;
Won Yong CHO
;
Hyoung Kyu KIM
;
Nan Hee WON
Author Information
1. Department of Internal Medicine, Eulji Medical College, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Ischemia/reperfusion injury;
Long- term sequelae;
alpha-MSH;
Reference
- MeSH:
Acute Kidney Injury;
alpha-MSH*;
Animals;
Apoptosis;
Constriction;
Creatinine;
Delayed Graft Function;
Fibrosis;
Humans;
Kidney Transplantation;
Kidney*;
Male;
Monocytes;
Proliferating Cell Nuclear Antigen;
Rats*;
Rats, Sprague-Dawley;
Reperfusion;
Reperfusion Injury
- From:Korean Journal of Nephrology
2005;24(2):191-203
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Ischemic acute renal failure (ARF) is increasingly recognized as involving chronic functional and structural sequelae. Ischemia reperfusion injury (I/R) plays a major role in delayed graft function and long-term changes after kidney transplantation, also. The present study was designed to evaluate the long-term changes after acute ischemic injury and whether renoprotection by alpha-MSH in the acute ischemic stage, could reduce the long-term sequelae. METHODS: In control group, ischemia/reperfusion injury was induced in male Sprague-Dawley rats by clamping Lt. renal pedicle for 15, 45, 60 minutes after removal of Rt. Kidney and followed by reperfusion. The animals in alpha-MSH group were injected alpha-MSH prior to reperfusion and then every day for 1 week. We measured BUN, creatinine at 24 hour after I/R injury, and in each group, 6 animals were sacrified at 1 week and 4 weeks after I/R injury to evaluate apoptosis, ED1, PCNA, and histopathologic changes. RESULTS: At 4 weeks after I/R injury, the remnant structural damage such as apoptosis, ED1 stained cells, MT (+) tubulointerstitial fibrosis were observed. alpha-MSH could reduce the initial functional injury, and apoptosis, ED1 stained monocyte, MT (+) tubulointerstitial fibrosis, also. CONCLUSION: Renal function was recoverd at 4 weeks after I/R injury, but structural sequelae such as apoptosis, fibrosis were remnant. alpha-MSH could attenuate initial functional damage and remnant fibrosis.
