Establishment and clinical application of time-resolved immunofluorometric assay for seurm CⅣ(collagenⅣ) measurement
- VernacularTitle:血清Ⅳ型胶原时间分辨荧光免疫分析检测法的建立和临床应用
- Author:
Xianghu JIANG
;
Hao PEI
;
Biao HUANG
;
Lan ZHU
;
Jinjuan QIAN
;
Ruiyun JI
;
Huimin WANG
- Publication Type:Journal Article
- Keywords:
time-resolved immunofluorometric assay;
CⅣ;
liver fibrosis
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a time-resolved immunofluorometric assay (TRFIA) to detect seurm CⅣ(collagenⅣ). Methods The antibodies to CⅣwere coated on mircoplate and the europium-labeled monoclonal antibody of CⅣ. The luminescent enhancement system was used as enhancement solution which contained mainly 2-naphthoy trifluoroacetone. we established A sandwich time-resolved fluoroimmunoassay (TRFIA) was established to measure the seurm CⅣin 127 patients with hepatitis and 30 normal controls. Results The sensitivity of assay was 12. 8?g/L. The coefficient of variation for inner-batch and inter-batch were 4. 54% and 8. 06%,respectively. The recovery was 98. 6%. The serum level of CⅣwas 46. 06?22. 21?g/L in normal control,47. 25?22. 58?g/L in acute hepatitis, 129.01?53.68?g/L in mild chronic hepatitis,277. 90?92.36?g/L in moderate chronic hepatitis,413.90?162.24?g/L in serious chronic hepatitis,568. 60?210.40?g/L in liver cirrhosis. As compared to normal control,higher concentrations of CIV (P
