The functions of GPⅠa/Ⅱa and GPⅣin platelet adhesion to collagen
- VernacularTitle:GPⅠa／Ⅱa和GPⅣ在血小板-胶原黏附中的功能
- Author:
Zhuo ZHANG
;
Yulong CONG
;
Zhiguang TU
- Publication Type:Journal Article
- Keywords:
platelet;
GPⅠa/Ⅱa;
collagen;
flow cytometry
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the functions of GPⅠa/Ⅱa and GPⅣduring the course of platelet adhesion to collagen. Methods Reaction system which contains anti-GPⅠa/Ⅱa antibody ,anti-GPⅣantibody or no blocking antibody was analyzed by flow cytomety. PE labeled anti-CD42b antibody was used to select platelet and fluorescence intension of fluorescein isothiocyanate ( FITC) was detected during the course of platelet adhesion to FITC-labeled collagen. Results The antibody against GPⅠa/Ⅱa inhibited platelet adhesion to collagen, especially to activated platelet(P 0. 05 ). Conclusion GPⅠa/Ⅱa plays an important roles in platelet adhesion to collagen. Blocking GPⅠa/Ⅱa may decrease the adhesion. GPⅣmay be helpful for acceleration of platelet adhesion to collagen at early stage.
