Protective Effect of Myocardial Ischemic Postconditioning on Ischemic-reperfused Myocardium of Rat and Its Relationship with P-Akt
- VernacularTitle:缺血后适应对大鼠缺血再灌注心肌的保护作用及其与P-Akt的关系
- Author:
Bo WANG
;
Demin AN
;
Qingzhi LI
;
Liang TAO
;
Nan ZHU
- Publication Type:Journal Article
- Keywords:
postconditioning;
myocardial protection;
ischemia-reperfusion;
phosphorylated protein kinase B
- From:
Journal of China Medical University
2010;(2):116-118,131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of the myocaidial ischemic postconditioning on ischemic-reperfused myocardium of rat and its signaling mechanism.Methods Healthy SD rats weighing 250 to 300 g were divided into blank control(N) group,ischemiareperfusion(IR) group and postconditioning(Post) group.We measured heart coronary perfusion flow and myocardial infarct size of the rats in each group.The expression of phosphorylated protein kinase B(P-Akt) was detected by Western blot.The morphological changes of myocardial fibers and mitochondrial were observed under the transmission electron microscopy.Results More coronary flow and less myocardial infarction were found in Post group than those in IR group.The expression level of P-Akt was higher in Post group.The integrity of myocardial fibers and mitochondrial was better in Post group than IR group.Conclusion Postconditioning had a protective effect for the ischemic isolated rat heart.Akt activation might play an important role in its mechinism.
