Effect of rhG-CSF mobilized peripheral blood and bone marrow after being mixed in different proportions on immunologic properties of grafts
- VernacularTitle:rhG-CSF动员的人外周血与骨髓不同比例混合对移植物免疫特性的影响
- Author:
Yingjun CHANG
;
Xiangyu ZHAO
;
Xiaojun HUANG
- Publication Type:Journal Article
- Keywords:
granulocyte colony-stimulating factor;
bone marrow;
peripheral blood stem cell;
immnunologic tolerance
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the immunologic composition and T cell function of rhG-CSF mobilized peripheral blood grafts(G-PB) and rhG-CSF primed bone marrow grafts(G-BM) after being mixed in different proportions.Methods T cell subgroups,dendritic cells(DC) and its subsets in G-PB and G-BM were analyzed by multicolor flow cytometry.Immunologic composition of G-PB and G-BM after mixture in different proportions were calculated,lymphocyte proliferation ability and quantities of interleukin-4(IL-4),interferon-?(IFN-?) secretion by T cells in mixture grafts were determined using MTT and sandwich ELISA assays.Results After co-incubation of G-PB with G-BM in different proportions,T cell proliferation in mixture grafts was lower than that of NG-BM and G-PB(P
