Effects of mobilized hematopoietic stem cells on the expression of C-FOS mRNA in focal cerebral ischemia/reperfusion rats
- VernacularTitle:动员造血干细胞对局灶性缺血再灌注大鼠脑组织C-FOS mRNA表达的影响
- Author:
Ziqiang ZHANG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia/reperfusion;
hematopoietic stem cells;
C-FOS
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of autologous hematopoietic stem cells mobilized by G-CSF on the expression of C-FOS mRNA in focal cerebral ischemia/reperfusion(MCAO/R) rats.Methods MCAO/R was induced by the filament occlusion method.Immunohistochemisty and dual labeling technique were used to detect HSCs differentiating into neuron-like cells after focal cerebral ischemia.RT-PCR technique was used to detect the expression of C-FOS mRNA.Results In the G-CSF treatment group,24 hours after the focal cerebral ischemia/reperfusion injury,CD34/Brdu dual labeled cells were found in the ischemia area;48 hours later,CD34/Brdu and CD34/NSE dual labeled cells were found,Compared with the control group,there was an enhanced expression level of C-FOS mRNA in the model group; In contrast,the expression of C-FOS mRNA in the G-CSF treatment group reduced.(Conclusion Autologous) hematopoietic stem cells mobilized by G-CSF can restore injury and decrease the expression of C-FOS mRNA.Some of HSCs can differentiate into neuron-like cells and may play an important role in the progress of the restoration.
