Determination of hypoxanthine and xanthine in Syngnathus by HPLC
- VernacularTitle:HPLC测定海龙中次黄嘌呤及黄嘌呤含量
- Author:
Xiaobing CUI
;
Wei LI
;
Kewei ZHANG
;
Hao CAI
;
Hao WU
- Publication Type:Journal Article
- Keywords:
Syngnathus;
hypoxanthine;
xanthine;
HPLC
- From:
Chinese Journal of Marine Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus.Methods A Lichrosper C_(18) Column was used.The mobile phase was methanol-0.1%HAc.Walvelength was 254nm.Results The linear range of hypoxanthine was within 5.91～94.56?g?mL~(-1),r=0.9999,sample recovery rate was 99.22%,RSD=(1.25)%.The linear range of Xanthine was within 2.04~32.64?g?mL~(-1),r=0.9998,sample recovery rate was 98.05%,RSD=1.21%.Conclusion This method has good repeatability and flexibility.It can be used for quality control in production of Syngnathus.
