Study of detection of EB virus in peripheral blood of patients with non-hodgkin lymphoma by ramification amplification method
- VernacularTitle:网状分枝扩增法检测非霍奇金淋巴瘤外周血中EB病毒基因的研究
- Author:
Fuming ZHANG
;
Fan LI
- Publication Type:Journal Article
- Keywords:
Herpes virus 4,human;
non-Hodgkin lymphoma/virology;
Ramification amplification method
- From:
Chinese Journal of Laboratory Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect EB virus in peripheral blood of patients with non-hodgkin lymphoma (NHL) by ramification amplification method (RAM).To investigate the relativity of NHL with infection of EB virus.Methods RAM was used to detect EB virus with the man-made target gene sequence of promotor of EBER-1. In 120 cases of patients with NHL, EB virus were detected by RAM, and the result of RAM was compared with that of PCR.Raji cells and NB4 cells were used as positive control and negative control respectively.Results RAM could detect 10 molecular target gene of the exponent.76 cases were positive in 120 cases of NHL patients and the positive rate was 63.3%, The result is identical with that of PCR.Conclusion Ramification amplification method is sensitive, convenient and easy to perform.NHL has a high relativity with infection caused by EB virus.
