Effects of mild hypothermia on heat shock protein 70 and glial fibrillary acidic protein expression after cerebral ischemia and reperfusion in rats
- VernacularTitle:亚低温对大鼠脑缺血再灌注损伤后热休克蛋白70及胶质纤维酸性蛋白表达的影响
- Author:
Xiuzhou ZHANG
;
Wenhua LI
;
Xueping LIU
- Publication Type:Journal Article
- Keywords:
mild hypothermia;
cerebral ischemia and reperfusion;
heat shock protein 70;
glial fibrillary acidic protein
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of mild hypothermia on heat shock protein 70 (HSP70) and glial fibrillary acidic protein (GFAP) expression in cerebral tissue after cerebral ischemia and reperfusion. Methods 30 male Wistar rats were randomly divided into sham-operated group, normal temperature group and mild hypothermia group. The model of focal cerebral ischemia was made by middle cerebral artery occlusion (MCAO) method. After 2 h of MCAO following 48 h of reperfusion, HSP70 and GFAP positive neurons were detected by immunohistochemistry technique in ischemic regions of rats. Results Many necrotic neurons were appeared in normal temperature group and no necrotic neurons in sham-operated group. Fewer necrotic neurons were fond in mild hypothermia group than in normal temperature group. The positive cells of HSP70 and GFAP in sham-operated group and mild hypothermia group were less than normal temperature group. Image analysis revealed that the average optical density of HSP70 and GFAP in sham-operated group and mild hypothermia group was significantly lower than in normal temperature group (all P
