Protective effects of Acanthopanax senticousus saponins on cortical neuronal ischemia-hypoxia injury
- VernacularTitle:刺五加皂甙对皮质神经元缺血缺氧性损伤的保护作用
- Author:
Yingzhu CHEN
;
Yongjian GU
;
Shiyao BAO
- Publication Type:Journal Article
- Keywords:
Acanthopanax senticousus saponins;
neuron;
ischemia;
glutamate;
nitric oxide
- From:
Journal of Clinical Neurology
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of Acanthopanax senticousus saponins (ASS) on ischemia-hypoxia injury of cortical neuron. Methods The models of cortical neuron damage induced by hypoxia-ischemia (HI) and glutamate (Glu) were established on cultured embryonic cortical neurons. The neurons were randomly divided into HI group, Glu group, ASS group and control group. ASS (50 mg/L) was added into the ASS group before and during neuron damage. The rate of neuronal apoptosis was measured by flow cytometer, nitric oxide (NO) content was determined by Nitrate reductive assay and neuron survival was measured by MTT assay and the release of LDH. Morphologic change of neurons was observed under electron microscopy.Results (1) The cortical neuron survival decreased time-dependently in HI group and reached peak at 8h after hypoxia-ischemia. Glutamate leaded the cortical neuron survival decreasing time-dependently. (2) Compared with the control group, the cortical neuron survival decreased in HI group and Glu group, but the neuron apoptosis, the release of LDH and NO contents increased significantly (all P
