Effects of CO_2 pneumoperitoneum on levels of interleukin-1? and interleukin-6,and peritoneal macrophages in tumor-bearing rats
- VernacularTitle:CO_2气腹对荷瘤大鼠IL-1?、IL-6和腹膜巨噬细胞功能的影响
- Author:
Qingjun GUO
;
Zhengjun QIU
;
Jun LIU
- Publication Type:Journal Article
- Keywords:
Interleukin-1?;
Interleukin-6;
Macrophage;
Tumor necrosis factor-?
- From:
Chinese Journal of Minimally Invasive Surgery
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of CO_2 pneumoperitoneum on cytokines and peritoneal macrophages in a rat model with implanted liver tumor.Methods A total of 32 Wistar rats with implanted liver tumor were randomly divided into 4 groups((n=8)): Control Group(anesthesia only),Laparotomy Group,Gasless Group(gasless laparoscopy),and Pneumoperitoneum Group(laparoscopy under CO_2 pneumoperitoneum).Serum samples were collected at the 2nd and 24th hours after the procedure respectively for the detection of levels of interleukin-1?(IL-1?) and interleukin-6(IL-6).Samples of peritoneal macrophages were collected and incubated for the detection of levels of tumor necrosis factor-?(TNF-?),a product of macrophages.Results At the 2nd and 24th hours after surgery,levels of serum IL-6 in the Laparotomy Group(57.92?2.06 pg/ml and 35.49?1.15 pg/ml) were significantly greater than those in the Pneumoperitoneum Group(14.64?0.34 pg/ml and 15.39?0.86 pg/ml),the Gasless Group(24.75?1.53 pg/ml and 17.10?0.97 pg/ml),and the Control Group(17.75?1.60 pg/ml and 14.55?0.25 pg/ml)(P
