In vitro protection of Jurkat cells from daunorubicin induced apoptosis by normal bone marrow stromal cells
- VernacularTitle:骨髓基质细胞对白血病细胞株的保护作用
- Author:
Zhongjun LI
;
Xinghua CHEN
;
Benxiu TENG
- Publication Type:Journal Article
- Keywords:
Bone marrow microenvironment;
Apoptosis;
Minimal residual leukemia
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of bone marrow microenvironment on leukemic cells during chemotherapy and the influence on leukemic cells' drug-resistance and anti-apoptosis. Methods Normal bone marrow stromal cells were isolated using Percoll, and then were cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. Apoptosis percentage of Jurkat cells labelled with Annexin V/PI were detected by FCM. Results Apoptosis percentage of Jurkat cells cocultured with bone marrow stromal cells for 24 hours was lower than those maintained in suspension (P
