Involvement of PI3K/Akt signaling in transforming growth factor-?_1 promoted epithelial-mesenchymal transdifferentiation of human kidney tubular epithelial cell
- VernacularTitle:磷脂酰肌醇3激酶/Akt通路参与TGF-?_1诱导人肾小管上皮细胞间质转分化过程
- Author:
Yong ZHANG
;
Jing ZHANG
- Publication Type:Journal Article
- Keywords:
Renal tubular epithelial cell;
Transdifferentiation;
Transforming growth factor-?_1;
Phosphatidylinositol 3-kinase;
Protein kinase B/Akt
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether the signaling of PI3K/Akt is involved in epithelial-mesenchymal transdifferentiation (EMT) of human kidney tubular epithelial cell (HKC) promoted by transforming growth factor (TGF)-?_1. Methods: the HKC was cultured on plastic plates in the presence or absence of recombinant human TGF-?_1 and PI3K inhibitor wortmannin. The expression of total- and phosphor-Akt and ?-SMA was assessed at different time point by Western blot. Results: In HKC, basic level of p-Akt, ?-SMA expression was found in the normal condition. Profound increase of p-Akt was seen at 0.5 h after induction of 10 ng/ml TGF-?_1 in HKC.?-SMA increased markedly at 48 h after the treatment of TGF-?_1. Adding PI3K inhibitor wortmannin (10 nmol/L) largely abrogated the effect of TGF-?_1. Wortmannin was shown to down-regulate ?-SMA and p-Akt expression in response to TGF-?_1. Conlusion: PI3K/Akt is a downstream mediator in EMT of HKC promoted by TGF-?_1, and PI3K inhibitor wortmannin can significantly inhibit EMT induced by TGF-?_1.
