Activity of nuclear factor-?B in the diabetic rat retina
- VernacularTitle:糖尿病大鼠视网膜病变核因子-?B表达水平的研究
- Author:
Hongjie DI
;
Ming ZHAO
;
Yangtian WANG
- Publication Type:Journal Article
- Keywords:
Nuclear factor-?B;
Retina;
Diabetes mellitus
- From:
Journal of Medical Postgraduates
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the express levels of transcription factor nuclear factor-?B in diabetic rat retina. Methods: Sprague-Dawley rats were randomly divided into control groups of 1, 3 and 6 months, diabetic groupsof 1, 3 and 6 months, respectively. Diabetic model of rat was induced by injecting streptozotocin (STZ). The retinal morphology was observed in paraffin slide by microscope. The expression of NF-?B was detected by immunohistochemistry and electrophoretic mobility shift assay. Results: Histopathological changes were detected at 3 and 6 months. The changes were severer at 3 months than at 6 months. Activation of retinal NF-?B was detected by electrophoretic mobility shift assay and immunohistochemistry at 1 month, and the NF-?B remained activated at 3 and 6 months in diabetic rats. Conclusion: NF-?B is activated in retina in diabetes before histopathological changes can be seen, and remains activated for the duration when the retinopathy is developing.
