ESBLs′ Genotypes of Escherichia coli and Klebsiella spp Isolated from Xinjiang: A Study
- VernacularTitle:新疆地区大肠埃希菌和克雷伯菌中产超广谱?-内酰胺酶基因型的研究
- Author:
Min DU
;
Xu CHEN
;
Shangzhu MA
;
Jiyun MA
;
Shulan ZHANG
;
Qiwen YANG
;
Yingchun XU
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Klebsiella spp;
ESBLs;
Genotype
- From:
Chinese Journal of Nosocomiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the resistant patterns of ESBLs-producing Escherichia coli and Klebsiella spp isolated from 3 hospitals in Xinjiang and to determine the genotypes of ESBLs. METHODS Using 2-fold agar dilution test to determine MICs of ten antibiotics.Using polymerase chain reaction and DNA sequencing to determine ESBLs genotype;using isoelectric focusing to determine the isoelectric points of ?-lactamases produced by strains in which no ESBLs genes were amplified by PCR. RESULTS Strains had low susceptibility to aztreonam,cefotaxime and ceftazidime.The susceptibility to cefepime,cefoperazone/sulbactam,cefmetazole,piperacillin/tazobactam,and meropenem were relatively high.Fifty five strains mainly carried blaCTX-M-1 subgroup gene and blaCTX-M-9subgroup gene,and blaSHV-12 gene were also detected. CONCLUSIONS blaCTX-M-1 Group gene and blaCTX-M-9 group gene are prevalent in three hospitals of Xinjiang,blaSHV-12 gene also plays a part role in leading to resistance of E.coli and Klebsiella spp to ?-lactams.
