Chromosomal and Subcellular Localization and Expression of Cell Cycle-related Regulator DCT1
- VernacularTitle:细胞周期相关蛋白DCT1染色体及细胞定位与其组织表达的研究
- Author:
Qi LIU
;
Shusen WANG
;
Jinlan GAO
;
Lihua CAO
;
Yang LUO
- Publication Type:Journal Article
- Keywords:
DCT1 gene;
DOC-1R gene;
chromosomal localization;
subcellular localization;
gene expression
- From:
Journal of China Medical University
2010;(2):105-107,111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the chromosomal and subcellular localization of DOC-1R terminal 1(DCT1),and detect its expression in human tissues.Methods Chromosome localization of DCT1 was detected by radiation hybrid.pEGFP-DCT1 was constructed,and HeLa cells were transfected with the plasmid.The subcellular localization of DCT1 protein was observed by fluorescence microscope.Real-time PCR was performed for the determination of DCT1 expression level in 16 kinds of human tissues.Results DCT1 was demonstrated to localize in 5q31,and its encoding protein was detected on the nuclear membrane.Additionally,DCT1 was proved to express universally in all the 16 kinds of human tissues and it was expressed at the highest level in spleen.Conclusion DCT1 might be a regulator in cell cycle,and ubiquitously express in human tissues.
