Tandem affinity purification technique: an extraordinary valuable tool for purifying protein complexes
- VernacularTitle:串联亲和纯化技术:一种极具潜力的分离、鉴定蛋白复合体的工具
- Author:
Wenna DI
;
Fang HUA
;
Xiaozhong PENG
- Publication Type:Journal Article
- Keywords:
TAP(tandem affinity purification);
protein complex
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
It's a multi-protein complex and not single protein that accomplish most of cellular activity.Therefore,to identify and analyze the composition of protein complex is necessary for studying the function of proteins.Tandem affinity purification technique(TAP) enables the effective purification of complex under physiological conditions without knowledge of the complex composition and function.Now,it has been adapted to analyze the composition of the protein complexes in yeast.Combined with mass spectrometry,TAP can identify the interacting proteins of target protein and offer great help for opening protein interacting network and function out.
