To optimize the method for inducing SW872 preadipocyte to differentiation
- VernacularTitle:诱导SW872前脂肪细胞分化的最优方法探讨
- Author:
Huiling LU
- Publication Type:Journal Article
- Keywords:
adipocyte;
differentiation;
oleic acid;
insulin
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a stable method to induce SW872 adipocyte to differentiation.Methods THE animals were randomly divided in to five groups according to different inducing agents and a control groups.The changes of the cells were observed by microscopy and Oil-Red O staining.TG mass of these cells were assayed by chemical colorimetry methods.Results The oleic acid is the strongest factor to induce SW872 cell to differentiate,IBMX+DEX+Insulin is also strong factor,but it took a longer time to act.The effect of IBMX is stronger than insulin and ASP,but less than Oleic acid and IBMX+DEX+Insulin.Oleic acid significantly increased triglyceride mass of SW872 cell on the 1~(th) day of differentiation,The TG mass in the oleic acid group increased on the 2~(th) and4~(th)day of differentiation. Conclusion Oleic acid is the best Inducer to stimulate SW872 cell to differentiate,it can induce SW872 preadipocytes to differentiate to adipicytes in a short term.
