A Progress in Detection of Interactions Between Macromolecules: Linked FRET Using Three Color Fluorophore
- VernacularTitle:生物大分子相互作用检测技术新进展——三色荧光级联荧光共振能量转移技术
- Author:
Chunchun LIU
;
Haiying HANG
- Publication Type:Journal Article
- Keywords:
fluorescence resonance energy transfer (FRET), confocal microscopy, flow cytometry
- From:
Progress in Biochemistry and Biophysics
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescence resonance energy transfer (FRET) is the energy transfer from an activated donor fluorophore to a receiving fluorophore. The efficiency of the energy transfer is the function of the distance between the two fluorophores, and is very sensitive to the distance. Its effective response distance is between 1~10 nm, thus it can be used to measure the distance between atoms or molecules. The feature of FRET is very useful in researches on conformational changes, interaction between macromolecules and signal transductions within live cells, and FRET has become a powerful tool in biomedical investigations. However, biological processes often involve interactions between more than two macromolecules, and FRET using two color fluorophores cannot meet the research demand. Recently, two research groups made breakthrough, establishing a novel FRET technique using three color fluorophores based on confocol microscopy and flow cytometry, respectively. The invention will significantly advance researches in biological and related fields.
