Construction and expression of inset expression vector pIZT/V5-His harboring mIL-4
- VernacularTitle:mIL-4昆虫表达载体pIZT/V5-His的构建及其高效表达
- Author:
Yanbo SUN
;
Jinghua LI
;
Hongyan SHI
- Publication Type:Journal Article
- Keywords:
interleukin-4;
insect cell Sf9;
gene expression;
genetic vectors
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish stable insect cell line expressing mIL4 on the basis of construction of inset expression vector pIZT/V5-His.Methods Amplified mIL-4 and pIZT/V5-His were treated with EcoRI and XbaI,and mIL-4 were ligated into pIZT/V5-His vector using T4 DNA ligase.Sf9 cells were transfected with recombinant DNA and ELISA was employed to detect soluble mIL-4 production by transfected Sf9 cells.Results Transfected Sf9 cells could significantly produce mIL4(1 mg?L~(-1)) compared!with control group(0.003 mg?L~(-1)).Conclusion Inset expression vector pIZT/V5-His is an ideal expression vector for mIL-4 production in transfected cells.
