Effects of human insulin-like growth factor 1 gene transfection on proliferation of NIH3T3 fibroblasts
- VernacularTitle:hIGF-1基因转染对成纤维细胞增殖的影响
- Author:
Shaokun ZHANG
;
Yan TAN
;
Yuxing SHAN
;
Zhiming SONG
;
Xinxiang XU
- Publication Type:Journal Article
- Keywords:
insulin-like growth factor 1;
gene expression;
fibroblasts;
3T3 cells;
cell cycle
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of human insulin-like growth factor 1(hIGF-1) gene transfection on the proliferation of NIH3T3 fibroblasts.Methods The plasmid of pcDNA3.1-hIGF-1 was transfected into NIH3T3 fibroblasts by using Lipofectin method.The positive cell clones were selected with G418 and cultured for 4 weeks.The stable expression of hIGF-1 in the positive cells was determined by in situ hybridization and immunocytochemical analysis.MTT assay and flow cytometer analysis were used to observe the proliferation of NIH3T3 fibroblasts.Results hIGF-1 mRNA and protein expressed in NIH3T3 fibroblasts transfected with pcDNA3.1-hIGF-1 by in situ hybridization and immunocytochemical analysis.MTT assay showed the A value of transfected NIH3T3 fibroblasts rose,compared with untransfected NIH3T3 fibroblasts group,the difference was significant(P
