Effect of Gross Saponin Tribulus Terrestris on protein kinase Cexpression in neonate rat cardiocytes injured by hypoxia
- VernacularTitle:蒺藜皂苷对缺氧心肌细胞内蛋白激酶C的作用
- Author:
Wei SUN
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
Gross Saponin Tribulus Terrestris;
protein kinase C;
laser confocal microscopy;
flow cytometry
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective mechanism of Gross Saponin Tribulus Terrestris(GSTT) on the neonatal rat ventricular cardiocytes injured by hypoxia and the effect on protein kinase C(PKC).Methods The hypoxia-ischemia model was performed by treating the cultured neonatal rat ventricular cardiocytes with NaCN.The effect of GSTT(100 and 30 mg?L~(-1)) on the contents of ?PKC and ?PKC were detected by flow cytometry and laser confocal microscopy system.Results GSTT up-regulated ?PKC and ?PKC expressions.The contents of ?PKC and ?PKC in GSTT 100 mg?L~(-1) group(1 325.00?53.25,810.55?36.89;66.22?6.23,40.12?2.21) were increased significantly than those in model group(792.00?32.36,492.40?30.15;32.70?2.78,29.28?4.82)(P
