Effect of taurine on primary cultured neonatal rat myocardial cells and cardiac fibroblasts
- VernacularTitle:牛磺酸对原代培养新生大鼠乳鼠心肌细胞和心肌成纤维细胞的作用
- Author:
Jing LI
;
Lijing ZHAO
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
taurine;
myocardium;
cells;
fibroblasts;
H_2O_2 damage
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of taurine on primary cultured neonatal rat myocardial cells and the inhibitory effect on cardiac fibroblast proliferation.Methods The myocardial cells were divided into six groups:control group,H_2O_2 damage group,H_2O_2 damage group treated with ligustrazine and H_2O_2 damage group treated with taurine with different doses(80,40 and 20 mmol?L~(-1)).Morpholoical changes of myocardial cells damaged for 6 h by 0.5 mmol?L ~(-1) H_2O_2 under the inverted microscope were observed.Meanwhile,cell survival ability and the contents of LDH,SOD and MDA were measured.Cardiac fibroblasts were randomly divided into(7 groups) and co-cultured for 72 h with taurine with doses of 0,10,20,40,80,160 and 320 mmol?L~(-1) and proliferation ability was assessed by MTT method.Results Absorption values in H_2O_2 damage groups treated with taurine(80,40 and 20 mmol?L~(-1)) were 0.479?0.055,0.437?0.052 and 0.421?0.062,respectively.They were higer than that in H_2O_2 damage group(0.304?0.050)(P
