Effects of 5-hydroxytryptamine on apoptosis of cultured human cytotrophoblast cells
- VernacularTitle:5-羟色胺对体外培养的人胎盘滋养细胞凋亡的影响
- Author:
Xiaojie LU
;
Mei WANG
- Publication Type:Journal Article
- Keywords:
trophoblast;
apoptosis;
serotonin
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of 5-hydroxytryptamine(5-HT) on apoptosis of cultured human cytotrophoblast cells and its possible mechanism involved in pathogenesis of pregnancy-induced hypertension syndrome.Methods Human cytotrophoblast cells from three term pregnancy(37-39 weeks) women were cultured and divided into four groups: normal control group,5-HT groups(0.1,1 and 10 ?mol?L~(-1)).The cells were cultivated for 3,6,12 and 24 h,respectively.Apoptotic index was measured by TdT-mediated biotinyated-dUTP nick end labeling(TUNEL) and morphological features of apoptotic cells were observed under electron microscope.Results When cells were cultivated for 3,6,12 and 24 h,compared with the normal control group(1.21?0.97,2.30?1.24,3.89?2.32 and 7.34?5.59,respectively),apoptotic indexes of cytotrophoblast cells in(10 ?mol?L~(-1)) 5-HT group were significantly increased(8.40?2.48,17.82?5.43,29.53?7.41,(45.56?)12.10,respectively)(P0.05).The significant morphological changes of apoptotic cytotrophoblast cells in various groups were found under electron microscope.Apoptotic cytotrophoblast cells were obviously compact and the chromatins were formed as mass and apoptotic bodies were also found under electron microscope.Conclusion 5-HT can promote the apoptosis of human cytotrophoblast cells and its mechanism may be correlated with pregnancy induced-hypertension syndrome.
