Apoptosis of cardiocytes of neonatal rats with simulated ischemia/reperfusion injury mediated by pro-apoptosis by protein Bid
- VernacularTitle:促凋亡蛋白Bid介导模拟缺血再灌注乳鼠心肌细胞的凋亡
- Author:
Shaojuan YU
;
Xuezhong ZHAO
;
Guocheng ZHANG
;
Li JI
- Publication Type:Journal Article
- Keywords:
Bid;
caspase-8;
reperfusion injury;
apoptosis;
RNA interference
- From:
Journal of Jilin University(Medicine Edition)
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Bid in simulated ischemia/reperfusion injury in cultured neonatal rat cardiocytes and its possible mechanism.Methods A cell culture model of neonatal rat(born within 3 days) cardiocytes was used to establish simulated ischemia/reperfusion model.Unrelated siRNA or Bid specific siRNA was transfected into rat cardiocytes by Oligofectamine.The activation of Bid and caspase-8 was detected by Western blotting.The apoptotic rate of cardiocytes was determined by flow cytometry.Results Bid and caspase-8 of cardiocytes were activatied in simulated ischemia/reperfusion injury model.The activation of caspase-8 of cardiocytes in Bid-specific siRNA group decreased significantly compared with those in simulated(ischemia/reperfusion) group and unrelated siRNA group;the apoptotic rate of cardrocytes(7.4%) was lower than those in simulated ischemia/reperfusion group(51.2%) and unrelated siRNA group(48.7%)(P
